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Total IgG fractions were purified from selected patients to evaluate their ability to inhibit prothrombin activation at low FII concentration.Īnticardiolipin antibodies (aCL) of the IgG or IgM type were found in 64 and 23 patients and LA activity in 49 patients. In LA patients, dependency of LA potency (as assessed by clotting time prolongation in absence or presence of hexagonal phospholipid) on autoantibody titers was analyzed by the generalized linear model. Odds ratios for the association of IgG binding activity with LA and the aCL IgG status were calculated. One hundred and ten consecutive patients with the clinical suspicion of APA syndrome (primary in 39) and 36 healthy controls were evaluated for the presence of LA activity (LA, Staclot, Stago), anticardiolipin antibodies (Quanta Lite aCL IgG, IgM, Inova Diagnostics), and IgG binding to solid-phase and/or phospholipid (PL)-bound beta(2)-GPI and FII by ELISA assays developed an optimized in our laboratory.
#Igg reactivity series
Because assays aimed at detecting LA activity are now commercially available, we evaluated the relative sensitivity to anti-FII and anti-beta(2)-GPI antibodies of a commercial LA assay in a consecutive series of patients with the clinical suspicion of anti-phospholipid antibody (APA) syndrome. However, LA activity sustained by anti-FII antibodies has a much weaker association with thrombosis than LA activity sustained by anti-beta(2)-GPI antibodies. If you need assistance with selecting the best format contact our expert technical support team.Autoantibodies to beta(2)-glycoprotein I (beta(2)-GPI) and/or prothrombin (FII) have been involved in the expression of lupus anticoagulant (LA) activity, an in vitro phenomenon associated with an increased risk of arterial and/or venous thromboembolic events. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. This data display is provided for general comparisons between formats. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.Ĩ999 BioLegend Way, San Diego, CA 92121 Toll-Free Phone: 1-877-Bio-Legend ( 246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587 By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
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Green Laser (532 nm)/Yellow-Green Laser (561 nm) It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. Do not freeze.Įach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The antibody solution should be stored undiluted between 2☌ and 8☌, and protected from prolonged exposure to light. The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.